| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Li, Emilia A (9)
|
MDEST |
41.12 |
40.10 |
-1.02 |
| 2 |
Piekutowski, Alia G (9)
|
MDNAAC |
41.97 |
41.22 |
-0.75 |
| 3 |
Hopkins, Madison E (9)
|
MDEST |
44.55 |
42.01 |
-2.54 |
| 4 |
Blaha, Adriana N (9)
|
MDEST |
43.86 |
42.85 |
-1.01 |
| 5 |
Greenwood, Allison H (10)
|
MDNAAC |
47.68 |
43.21 |
-4.47 |
| 6 |
Ryan, Erin A (9)
|
MDEST |
45.52 |
43.23 |
-2.29 |
| 7 |
Kirchen, Trinity M (9)
|
MDNAAC |
44.93 |
43.68 |
-1.25 |
| 8 |
Parkinson, Scarlett K (10)
|
MDNAAC |
52.65 |
44.42 |
-8.23 |
| 9 |
Nicholson, Riley A (10)
|
MDEST |
46.29 |
45.71 |
-0.58 |
| 10 |
Leong, Katrina (9)
|
MDEST |
48.21 |
45.96 |
-2.25 |
| 11 |
Temple, Alexandra V (10)
|
MDEST |
49.20 |
47.01 |
-2.19 |
| 12 |
Santiago, Genevieve M (9)
|
MDNAAC |
53.48 |
51.52 |
-1.96 |
| 13 |
Sullivan, Riley M (10)
|
MDNAAC |
1:04.73 |
51.72 |
-13.01 |
| 14 |
Samuel, Emily J (9)
|
MDEST |
49.86 |
52.51 |
2.65 |
| 15 |
O'Connor, Margaret A (9)
|
MDNAAC |
1:06.52 |
53.07 |
-13.45 |
|
Ku, Evelyn D (9)
|
MDEST |
43.71 |
DQ |
- |
|
Alexander, Emma C (10)
|
MDEST |
49.42 |
NS |
- |